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Effects of low-frequency hippocampal stimulation on GABA-B receptor expression in pharmacoresistant amygdaloid kindling epileptic rats

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Effects of low-frequency hippocampal stimulation on GABA-B receptor expression in pharmacoresistant amygdaloid kindling epileptic rats 
1,2Guofeng Wu, 2,*Zhen Hong, 1Yan Li,1 Feng Zhou, 1Jing Shi
1 Department of Neurology, Affiliated Hospital, Guiyang Medical College, Guiyang City, Guizhou Province, PRC 550004
Postal Address of Authors: No. 28, Guiyi jie Road, Liuguangmen, Guiyang City, Guizhou Province, PRC, Postal code 550004
2 Department of Neurology, Affiliated Huashan Hospital, Fudan University, Shanghai City, Shanghai, PRC 200040
Postal Address of Corresponding author: No.12, Urumuqi(Middle) Road, Shanghai, China, Postal code 200040
*Corresponding Author: Zhen Hong; TEL: +86-1391-6666-552, E-mail address: Hongzhen_fudan@sina.com, Fax number: +86-021-6248-1930
Abstract
Objective: To observe the effect of low-frequency hippocampal stimulation on GABA-B receptor expression in hippocampus pharmacoresistant epileptic rats. Materials and Methods: Sixteen pharmacoresistant epileptic rats were selected by testing their seizure response to phenytoin and phenobarbital, and they were randomly divided into a pharmacoresistant control group (PRC group, 8 rats) and a pharmacoresistant stimulation group (PRS group, 8 rats). Another 16 pharmacosensitive epileptic rats were served as control, also divided randomly into a pharmacosensitive control group (PSC group) and a pharmacosensitive stimulation group (PSS group). A stimulation electrode was implanted into the rats` hippocampus in the four groups. Low frequency hippocampal stimulation was administered twice per day for two weeks. Following these weeks of stimulation, GABA-B receptor-positive neurons were counted and the gray values of GABA-B receptor expression in the rats` hippocampal tissues were measured. Results: The amygdale stimulus-induced epileptic seizures were decreased significantly in the PRS group compared with the PRC group. The parameters of the amygdale afterdischarge were also improved after hippocampal stimulation. Simultaneously, the GABA-B receptor-positive neurons increased and the GABA-B expression gray values decreased markedly in the PRS group compared to the PRC group. The same phenomenon was also observed between the PSS group and the PSC group. However, no significant difference was found in the GABA-B receptor-positive neurons and the gray values of GABA-B between the PRS group and the PSC group.Conclusions: The low frequency hippocampal stimulation may inhibit the amygdale stimulus-induced epileptic seizures and the afterdischarges. The antiepileptic effects of the hippocampal stimulation may be achieved partly by increasing the expression of the GABA-B receptor.
Key Words: Pharmacoresistant epilepsy; Hippocampal stimulation; GABA-B receptor; Deep brain stimulation; Kindling epileptic models; Amygdala